Antichymotrypsin interaction with chymotrypsin: Identification of intermediates on the path to inhibited complex
Derivatization of both serpin and proteinase with fluorophores allows for the reaction to be monitored by FRET as well as by simple changes in fluorescence. A Cys variant of rACT at P1' was derivatized with BMMC. Chymotrypsin was also labeled with the succinimidyl ester of 7-diethylaminocoumari...
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| Format: | Thesis Book |
| Language: | English |
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