Transgenic plants : methods and protocols /
A collection of readily reproducible techniques for the genetic transformation and regeneration of plants. The authors cover the most commonly used transformation systems for the regeneration of whole transgenic plants. Among the techniques discussed for the detection of transgenes are quantitative...
| Other Authors: | , |
|---|---|
| Format: | Book |
| Language: | English |
| Published: |
Totowa, N.J. :
Humana Press,
2005
Totowa, N.J. : c2005 Totowa, N.J. : ©2005 Totowa, NJ : 2005 Totowa, NJ : 2005 |
| Series: | Methods in molecular biology (Clifton, N.J.) ;
v. 286 Methods in molecular biology (Clifton, N.J.) 286 Methods in molecular biology ; v. 286 |
| Subjects: |
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| 245 | 0 | 0 | |a Transgenic plants : |b methods and protocols / |c edited by Leandro Peña |
| 260 | |a Totowa, N.J. : |b Humana Press, |c 2005 | ||
| 260 | |a Totowa, N.J. : |b Humana Press, |c c2005 | ||
| 260 | |a Totowa, N.J. : |b Humana Press, |c ©2005 | ||
| 260 | |a Totowa, NJ : |b Humana Press, |c 2005 | ||
| 264 | 1 | |a Totowa, NJ : |b Humana Press, |c 2005 | |
| 300 | |a xiv, 437 p. : |b ill. ; |c 24 cm | ||
| 300 | |a xiv, 437 p. : |b ill. (some col.) ; |c 24 cm | ||
| 300 | |a xiv, 437 p. : |b ill. ; |c 24 cm | ||
| 300 | |a xiv, 437 pages : |b illustrations (some color) ; |c 24 cm | ||
| 300 | |a xiv, 437 pages : |b illustrations ; |c 24 cm | ||
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| 337 | |a unmediated |b n |2 rdamedia | ||
| 338 | |a volume |b nc |2 rdacarrier | ||
| 490 | 1 | |a Methods in molecular biology ; |v 286 | |
| 490 | 1 | |a Methods in molecular biology ; |v v. 286 | |
| 500 | |a This WorldCat-derived record is shareable under Open Data Commons ODC-BY, with attribution to OCLC |5 CTY | ||
| 504 | |a Includes bibliographical references and index | ||
| 504 | |a Includes bibliographical references | ||
| 505 | 0 | |a Analysis of gene expression in transgenc plants / Andrew F. Page and Subhash C. Minocha -- Transgene integration: use of matrix attachment regions / George C. Allen, Steven Spiker, and William F. Thompson -- Fluorescence in situ hybridization to localize transgenes in plant chromosomes / Wendy A. Harwood [and others] -- Amplification of genomic sequences flanking T-DNA insertions by thermal asymmetric interlaced polymerase chain reaction / Yao-Guang Liu, Yuanlin Chen, and Qunyu Zhang -- Agrobacterium persistence in plant tissues after transformation / Jaime Cubero and María M. López -- Transgene dispersal through pollen / Laura C. Hudson, Matthew D. Halfhill, and C. Neal Stewart, Jr. -- Transgenic crops: the current and next generations / Jim M. Dunwell -- Comparative development and impact of transgenic papayas in Hawaii, Jamaica, and Venezuela / Gustavo Fermín [and others] | |
| 505 | 0 | |a Genetic transformation of conifers utilizing somatic embryogenesis / Krystyna Klimaszewska, Robert G. Rutledge, and Armand Séguin -- Regeneration of transgenic cassava from transformed embryogenic tissues / Peng Zhang and Johanna Puonti-Kaerlas -- Genetic transformation of mature citrus plants / Magdalena Cervera [and others] -- Selectable markers: antibiotic and herbicide resistance / Julia L. Goodwin [and others] -- Histochemical and fluorometric assays for uidA (GUS) gene detection / Magdalena Cervera -- Green fluorescent protein quantification in whole plants / Matthew D. Halfhill, Reginald J. Millwood, and C. Neal Stewart, Jr. -- Positive selection / Allan Wenck and Geneviève Hansen -- Elimination of marker genes from transgenic plants using MAT vector systems / Hiroyasu Ebinuma [and others] -- Simple and efficient removal of marker genes from plastids by homologous recombination / Anil Day [and others] -- The study of transgene copy number and organization / David J. Ingham --^ | |
| 505 | 0 | |a Transgenic plants: an historical perspective / Luis Herrera-Estrella, June Simpson, and Miguel Martínez-Trujillo -- Plant transformation: agrobacterium-mediated gene transfer / Abhaya M. Dandekar and Henry J. Fisk -- Production of hairy root cultures and transgenic plants by Agrobacterium rhizogenes-mediated transformation / Mary C. Christey and Robert H. Braun -- Stable transformation of plant cells by particle bombardment/biolistics /Julie R. Kikkert, José R. Vidal, and Bruce I. Reisch -- Electroporation: introduction and expression of transgenes in plant protoplasts / Henry J. Fisk and Abhaya M. Dandekar -- Floral dip: agrobacterium-mediated germ line transformation / Steven J. Clough -- Production of transgenic crops by the floral-dip method / Ian S. Curtis -- Chloroplast genetic engineering to improve agronomic traits / Henry Daniell, Oscar N. Ruiz, and Amit Dhingra -- Organogenesis from transformed tomato explants / Anne Frary and Joyce Van Eck -- Genetic transformation of conifers utilizing somatic embryogenesis / Krystyna Klimaszewska, Robert G. Rutledge, and Armand Séguin -- Regeneration of transgenic cassava from transformed embryogenic tissues / Peng Zhang and Johanna Puonti-Kaerlas -- Genetic transformation of mature citrus plants / Magdalena Cervera [and others] -- Selectable markers: antibiotic and herbicide resistance / Julia L. Goodwin [and others] -- Histochemical and fluorometric assays for uidA (GUS) gene detection / Magdalena Cervera -- Green fluorescent protein quantification in whole plants / Matthew D. Halfhill, Reginald J. Millwood, and C. Neal Stewart, Jr. -- Positive selection / Allan Wenck and Geneviève Hansen -- Elimination of marker genes from transgenic plants using MAT vector systems / Hiroyasu Ebinuma [and others] -- Simple and efficient removal of marker genes from plastids by homologous recombination / Anil Day [and others] -- The study of transgene copy number and organization / David J. Ingham -- Analysis of gene expression in transgenc plants / Andrew F. Page and Subhash C. Minocha -- Transgene integration: use of matrix attachment regions / George C. Allen, Steven Spiker, and William F. Thompson -- Fluorescence in situ hybridization to localize transgenes in plant chromosomes / Wendy A. Harwood [and others] -- Amplification of genomic sequences flanking T-DNA insertions by thermal asymmetric interlaced polymerase chain reaction / Yao-Guang Liu, Yuanlin Chen, and Qunyu Zhang -- Agrobacterium persistence in plant tissues after transformation / Jaime Cubero and María M. López -- Transgene dispersal through pollen / Laura C. Hudson, Matthew D. Halfhill, and C. Neal Stewart, Jr. -- Transgenic crops: the current and next generations / Jim M. Dunwell -- Comparative development and impact of transgenic papayas in Hawaii, Jamaica, and Venezuela / Gustavo Fermín [and others] | |
| 505 | 0 | |a Transgenic plants: an historical perspective / Luis Herrera-Estrella, June Simpson, and Miguel Martínez-Trujillo -- Plant transformation: agrobacterium-mediated gene transfer / Abhaya M. Dandekar and Henry J. Fisk -- Production of hairy root cultures and transgenic plants by Agrobacterium rhizogenes-mediated transformation / Mary C. Christey and Robert H. Braun -- Stable transformation of plant cells by particle bombardment/biolistics /Julie R. Kikkert, José R. Vidal, and Bruce I. Reisch -- Electroporation: introduction and expression of transgenes in plant protoplasts / Henry J. Fisk and Abhaya M. Dandekar -- Floral dip: agrobacterium-mediated germ line transformation / Steven J. Clough -- Production of transgenic crops by the floral-dip method / Ian S. Curtis -- Chloroplast genetic engineering to improve agronomic traits / Henry Daniell, Oscar N. Ruiz, and Amit Dhingra -- Organogenesis from transformed tomato explants / Anne Frary and Joyce Van Eck --^ | |
| 505 | 0 | 0 | |g 1 |t Transgenic Plants: An Historical Perspective / |r Luis Herrera-Estrella, June Simpson, Miguel Martinez-Trujillo |g 3 -- |g Part II. |t Transformation -- |g 2. |t Plant Transformation: Agrobacterium-Mediated Gene Transfer / |r Abhaya M. Dandekar, Henry J. Fisk |g 35 -- |g 3. |t Production of Hairy Root Cultures and Transgenic Plants by Agrobacterium rhizogenes-Mediated Transformation / |r Mary C. Christey, Robert H. Braun |g 47 -- |g 4. |t Stable Transformation of Plant Cells by Particle Bombardment/Biolistics / |r Julie R. Kikkert, Jose R. Vidal, Bruce I. Reisch |g 61 -- |g 5. |t Electroporation: Introduction and Expression of Transgenes in Plant Protoplasts / |r Henry J. Fisk, Abhaya M. Dandekar |g 79 -- |g 6. |t Floral Dip: Agrobacterium-Mediated Germ Line Transformation / |r Steven J. Clough |g 91 -- |g 7. |t Production of Transgenic Crops by the Floral-Dip Method / |r Ian S. Curtis |g 103 -- |g 8. |t Chloroplast Genetic Engineering to Improve Agronomic Traits / |r Henry Daniell, Oscar N. Ruiz, Amit Dhingra |g 111 -- |g Part III. |t Regeneration -- |g 9. |t Organogenesis From Transformed Tomato Explants / |r Anne Frary, Joyce Van Eck |g 141 -- |g 10. |t Genetic Transformation of Conifers Utilizing Somatic Embryogenesis / |r Krystyna Klimaszewska, Robert G. Rutledge, Armand Seguin |g 151 -- |g 11. |t Regeneration of Transgenic Cassava From Transformed Embryogenic Tissues / |r Peng Zhang, Johanna Puonti-Kaerlas |g 165 -- |g 12. |t Genetic Transformation of Mature Citrus Plants / |r Magdalena Cervera, Jose Juarez, Luis Navarro, Leandro Pena |g 177 -- |g Part IV. |t Selection -- |g 13. |t Selectable Markers: Antibiotic and Herbicide Resistance / |r Julia L. Goodwin, Gabriela M. Pastori, Michael R. Davey, Huw D. Jones |g 191 -- |g 14. |t Histochemical and Fluorometric Assays for uidA (GUS) Gene Detection / |r Magdalena Cervera |g 203 -- |g 15. |t Green Fluorescent Protein Quantification in Whole Plants / |r Matthew D. Halfhill, Reginald J. Millwood, C. Neal Steart, Jr. |g 215 -- |g 16. |t Positive Selection / |r Allan Wenck, Genevieve Hansen |g 227 -- |g 17. |t Elimination of Marker Genes From Transgenic Plants Using MAT Vector Systems / |r Hiroyasu Ebinuma, Koichi Sugita, Saori Endo, Etsuko Matsunaga, Keiko Yamada |g 237 -- |g 18. |t Simple and Efficient Removal of Marker Genes From Plastids by Homologous Recombination / |r Anil Day, Vasumathi Kode, Panagiotis Madesis, Siriluck Iamtham |g 255 -- |g Part V. |t Transgene Integration, Expression, and Localization -- |g 19. |t The Study of Transgene Copy Number and Organization / |r David J. Ingham |g 273 -- |g 20. |t Analysis of Gene Expression in Transgenic Plants / |r Andrew F. Page, Subhash C. Minocha |g 291 -- |g 21. |t Transgene Integration: Use of Matrix Attachment Regions / |r George C. Allen, Steven Spiker, William F. Thompson |g 313 -- |g 22. |t Fluorescence In Situ Hybridization to Localize Transgenes in Plant Chromosomes / |r Wendy A. Harwood, Lorelei J. Bilham, Silvia Travella, Haroldo Salvo-Garrido, John W. Snape |g 327 -- |g 23. |t Amplification of Genomic Sequences Flanking T-DNA Insertions by Thermal Asymmetric Interlaced Polymerase Chain Reaction / |r Yao-Guang Liu, Yuanlin Chen, Qunyu Zhang |g 341 -- |g Part VI. |t Risk Assessment -- |g 24. |t Agrobacterium Persistence in Plant Tissues After Transformation / |r Jaime Cubero, Maria M. Lopez |g 351 -- |g 25. |t Transgene Dispersal Through Pollen / |r Laura C. Hudson, Matthew D. Halfhill, C. Neal Stewart, Jr. |g 365 -- |g Part VII. |t Transgenic Crops -- |g 26. |t Transgenic Crops: The Current and Next Generations / |r Jim M. Dunwell |g 377 -- |g 27. |t Comparative Development and Impact of Transgenic Papayas in Hawaii, Jamaica, and Venezuela / |r Gustavo Fermin, Paula Tennant, Carol Gonsalves, David Lee, Dennis Gonsalves |g 399. |
| 520 | |a A collection of readily reproducible techniques for the genetic transformation and regeneration of plants. The authors cover the most commonly used transformation systems for the regeneration of whole transgenic plants. Among the techniques discussed for the detection of transgenes are quantitative real-time PCR, RT-PCR, in situ hybridization, FISH, and TAIL-PCR. Risk assessment methods offer the opportunity to study Agrobacterium persistence in plant tissues and to investigate the possibility of transgene dispersal. The protocols follow the successful Methods in Molecular Biology??? series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls | ||
| 530 | |a Also available in an electronic version | ||
| 530 | |a Also available on the Internet to registered users | ||
| 590 | |a OCLC |b WorldCat Holdings | ||
| 590 | |a WELCH: Held in Web version only | ||
| 650 | 0 | |a Transgenic plants |v Laboratory manuals |0 http://id.loc.gov/authorities/subjects/sh99001586 | |
| 650 | 0 | |a Transgenic plants |v Laboratory manuals | |
| 650 | 2 | |a Genetic Engineering |x methods | |
| 650 | 4 | |a Transgenic plants |x Laboratory manuals | |
| 650 | 7 | |a Melhoramento genético vegetal |2 larpcal | |
| 650 | 7 | |a Plantas transgênicas |2 larpcal | |
| 650 | 7 | |a Transgenic plants |2 fast | |
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| 650 | 0 | 7 | |a Transgene Pflanzen |2 swd |
| 650 | 1 | 2 | |a Plants, Genetically Modified |v Laboratory Manuals |
| 650 | 1 | 2 | |a Plants, Genetically Modified |
| 650 | 2 | 2 | |a Biotechnology |
| 650 | 2 | 2 | |a Gene Expression Regulation, Plant |
| 650 | 2 | 2 | |a Genetic Engineering |x methods |
| 650 | 2 | 2 | |a Genetic Engineering |
| 655 | 2 | |a Laboratory Manual |0 http://id.nlm.nih.gov/mesh/D020484 | |
| 655 | 2 | |a Laboratory Manual | |
| 655 | 2 | |a Laboratory Manuals | |
| 655 | 4 | |a Aufsatzsammlung | |
| 655 | 7 | |a Aufsatzsammlung |2 swd | |
| 655 | 7 | |a Handbooks and manuals |2 fast | |
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| 655 | 7 | |a Handbooks, manuals, etc |2 fast | |
| 655 | 7 | |a Laboratory Manuals |2 fast | |
| 655 | 7 | |a Laboratory manuals |2 fast |0 http://id.worldcat.org/fast/1920776 | |
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| 700 | 1 | |a Peña, Leandro |0 http://viaf.org/viaf/73239597 | |
| 700 | 1 | |a Peña, Leandro |1 http://viaf.org/viaf/73239597 | |
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