Drosophila : methods and protocols /
With its long-standing tradition as a model organism, many techniques using Drosophila melanogaster have been established and continue to be developed, while the recent invention of techniques allowing the knock-down of genes by RNA interference and gene replacement by homologous recombination demon...
| Other Authors: | |
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| Format: | Book |
| Language: | English |
| Published: |
Totowa, N.J. : London :
Humana ; Springer [distributor],
2008
Totowa, N.J. : [London : Humana Press ; Springer, distributor], c2008 Totowa, N.J. : Humana Press, c2008 Totowa, N.J. : [London] : Humana Press ; [Springer, distributor], [2008] |
| Series: | Methods in molecular biology (Clifton, N.J.) ;
420 Methods in molecular biology (Clifton, N.J.) ; v. 420 Methods in molecular biology (Clifton, N.J.) 420 Methods in molecular biology ; v. 420 Methods in molecular biology v. 420 |
| Subjects: |
Table of Contents:
- Drosophila melanogaster and the development of biology in the 20th century / Alfonso Martinez Arias
- Getting started : an overview on raising and handling Drosophila / Hugo Stocker and Peter Gallant
- FlyBase : a database for the Drosophila research community / Rachel Drysdale and the FlyBase Consortium
- The use of P-element transposons to generate transgenic flies / André Bachmann and Elisabeth Knust
- The GAL4 system : a verstile system for the expression of genes / David A. Elliott and Andrea H. Brand
- P-element mutagenesis / Thomas Hummel and Christian Klämbt
- EMS screens : from mutagenesis to screening and mapping / Christian Bökel
- RNAi screening in cultured Drosophila cells / Sandra Steinbrink and Michael Boutros
- Methods for homologous recombination in Drosophila / Keith A. Maggert, Wei J. Gong, and Kent G. Golic
- Recombinases and their use in gene activation, gene inactivation, and transgenesis / Johannes Bischof and Konrad Basler
- Cuticle preparation of Drosophila embryos and larvae / Cyrille Alexandre
- Immunolabeling of embryos / H.-Arno J. Müller
- Imaging cellular and molecular dynamics in live embryos using fluorescent proteins / Matthieu Cavey and Thomas Lecuit
- Analysis of neuromuscular junctions : histology and in vivo imaging / Andreas Schmid and Stephan J. Sigrist
- Immunolabeling of imaginal discs / Thomas Klein
- Imaging Drosophila pupal wing morphogenesis / Anne-Kathrin Classen ... [et al.]
- Microscopic analysis of the adult Drosophila retina using semithin plastic section / Konstantin Gaengel and Marek Mlodzik
- Fluorescent in situ hybridization protocols in Drosophila embryos and tissues / Eric Lécuyer, Neela Parthasarathy, and Henry M. Krause
- Linear RNA amplification for the production of microarray hybridization probes / Ansgar Klebes and Thomas B. Kornberg
- An overview of the identification, detection, and functional analysis of Drosophila microRNAs / Nicholas S. Sokol
- Extraction and immunoblotting of proteins from embryos / Andreas Wodarz
- Purification of Drosophila protein complexes for mass spectrometry / Christoph Jüschke and Jürgen A. Knoblich
- Mass production of Drosophila embryos and chromatographic purification of native protein complexes / Natascha Kunert and Alexander Brehm
- Flow cytometric analysis of Drosophila cells / Aida Flor A. de la Cruz and Bruce A. Edgar
- Drosophila cell lines as model systems and as an experimental tool / Buzz Baum and Lucy Cherbas
- Appendix : phenotypic markers in Drosophila / Jennifer Childress and Georg Halder
- Drosophila melanogaster and the development of biology in the 20th century / Alfonso Martinez Arias
- Getting started : an overview on raising and handling Drosophila / Hugo Stocker and Peter Gallant
- FlyBase : a database for the Drosophila research community / Rachel Drysdale and the FlyBase Consortium
- The use of P-element transposons to generate transgenic flies / André Bachmann and Elisabeth Knust
- The GAL4 system : a verstile system for the expression of genes / David A. Elliott and Andrea H. Brand
- P-element mutagenesis / Thomas Hummel and Christian Klämbt
- EMS screens : from mutagenesis to screening and mapping / Christian Bökel
- RNAi screening in cultured Drosophila cells / Sandra Steinbrink and Michael Boutros
- Methods for homologous recombination in Drosophila / Keith A. Maggert, Wei J. Gong, and Kent G. Golic
- Recombinases and their use in gene activation, gene inactivation, and transgenesis / Johannes Bischof and Konrad Basler
- Cuticle preparation of Drosophila embryos and larvae / Cyrille Alexandre
- Immunolabeling of embryos / H.-Arno J. Müller
- Imaging cellular and molecular dynamics in live embryos using fluorescent proteins / Matthieu Cavey and Thomas Lecuit
- Analysis of neuromuscular junctions : histology and in vivo imaging / Andreas Schmid and Stephan J. Sigrist
- Immunolabeling of imaginal discs / Thomas Klein
- Imaging Drosophila pupal wing morphogenesis / Anne-Kathrin Classen ... [et al.]
- Microscopic analysis of the adult Drosophila retina using semithin plastic section / Konstantin Gaengel and Marek Mlodzik
- Fluorescent in situ hybridization protocols in Drosophila embryos and tissues / Eric Lécuyer, Neela Parthasarathy, and Henry M. Krause
- Linear RNA amplification for the production of microarray hybridization probes / Ansgar Klebes and Thomas B. Kornberg
- An overview of the identification, detection, and functional analysis of Drosophila microRNAs / Nicholas S. Sokol
- Extraction and immunoblotting of proteins from embryos / Andreas Wodarz
- Purification of Drosophila protein complexes for mass spectrometry / Christoph Jüschke and Jürgen A. Knoblich
- Mass production of Drosophila embryos and chromatographic purification of native protein complexes / Natascha Kunert and Alexander Brehm
- Flow cytometric analysis of Drosophila cells / Aida Flor A. de la Cruz and Bruce A. Edgar
- Drosophila cell lines as model systems and as an experimental tool / Buzz Baum and Lucy Cherbas
- Appendix : phenotypic markers in Drosophila / Jennifer Childress and Georg Halder
- Drosophila melanogaster and the development of biology in the 20th century / Alfonso Martinez Arias
- Getting started : an overview on raising and handling Drosophila / Hugo Stocker and Peter Gallant
- FlyBase : a database for the Drosophila research community / Rachel Drysdale and the FlyBase Consortium
- The use of P-element transposons to generate transgenic flies / André Bachmann and Elisabeth Knust
- The GAL4 system : a verstile system for the expression of genes / David A. Elliott and Andrea H. Brand
- P-element mutagenesis / Thomas Hummel and Christian Klämbt
- EMS screens : from mutagenesis to screening and mapping / Christian Bökel
- 1 Drosophila melanogaster and the Development of Biology in the 20th Century / Alfonso Martinez Arias
- 2. Getting Started: An Overview on Raising and Handling Drosophila / Hugo Stocker and Peter Gallant
- 3. FlyBase: A Database for the Drosophila Research Community / Rachel Drysdale / the FlyBase Consortium
- 4. The Use of P-Element Transposons to Generate Transgenic Flies / Andre Bachmann and Elisabeth Knust
- 5. The GAL4 System: A Versatile System for the Expression of Genes / David A. Elliott and Andrea H. Brand
- 6. P-Element Mutagenesis / Thomas Hummel and Christian Klambt
- 7. EMS Screens: From Mutagenesis to Screening and Mapping / Christian Bokel
- 8. RNAi Screening in Cultured Drosophila Cells / Sandra Steinbrink and Michael Boutros
- 9. Methods for Homologous Recombination in Drosophila / Keith A. Maggert, Wei J. Gong and Kent G. Golic
- 10. Recombinases and Their Use in Gene Activation, Gene Inactivation, and Transgenesis / Johannes Bischof and Konrad Basler
- 11. Cuticle Preparation of Drosophila Embryos and Larvae / Cyrille Alexandre
- 12. Immunolabeling of Embryos / H.-Arno J. Muller
- 13. Imaging Cellular and Molecular Dynamics in Live Embryos Using Fluorescent Proteins / Matthieu Cavey and Thomas Lecuit
- 14. Analysis of Neuromuscular Junctions: Histology and In Vivo Imaging / Andreas Schmid and Stephan J. Sigrist
- 15. Immunolabeling of Imaginal Discs / Thomas Klein
- 16. Imaging Drosophila Pupal Wing Morphogenesis / Anne-Kathrin Classen, Benoit Aigouy, Angela Giangrande and Suzanne Eaton
- 17. Microscopic Analysis of the Adult Drosophila Retina Using Semithin Plastic Sections / Konstantin Gaengel and Marek Mlodzik
- 18. Fluorescent In Situ Hybridization Protocols in Drosophila Embryos and Tissues / Eric Lecuyer, Neela Parthasarathy and Henry M. Krause
- 19. Linear RNA Amplification for the Production of Microarray Hybridization Probes / Ansgar Klebes and Thomas B. Kornberg
- 20. An Overview of the Identification, Detection, and Functional Analysis of Drosophila MicroRNAs / Nicholas S. Sokol
- 21. Extraction and Immunoblotting of Proteins From Embryos / Andreas Wodarz
- 22. Purification of Drosophila Protein Complexes for Mass Spectrometry / Christoph Juschke and Jurgen A. Knoblich
- 23. Mass Production of Drosophila Embryos and Chromatographic Purification of Native Protein Complexes / Natascha Kunert and Alexander Brehm
- 24. Flow Cytometric Analysis of Drosophila Cells / Aida Flor A. de la Cruz and Bruce A. Edgar
- 25. Drosophila Cell Lines as Model Systems and as an Experimental Tool / Buzz Baum and Lucy Cherbas
- Appendix. Phenotypic Markers in Drosophila / Jennifer Childress and Georg Halder.
- Extraction and immunoblotting of proteins from embryos / Andreas Wodarz
- Purification of Drosophila protein complexes for mass spectrometry / Christoph Jüschke and Jürgen A. Knoblich
- Mass production of Drosophila embryos and chromatographic purification of native protein complexes / Natascha Kunert and Alexander Brehm
- Flow cytometric analysis of Drosophila cells / Aida Flor A. de la Cruz and Bruce A. Edgar
- Drosophila cell lines as model systems and as an experimental tool / Buzz Baum and Lucy Cherbas
- Appendix : phenotypic markers in Drosophila / Jennifer Childress and Georg Halder
- Immunolabeling of imaginal discs / Thomas Klein
- Imaging Drosophila pupal wing morphogenesis / Anne-Kathrin Classen ... [et al.]
- Microscopic analysis of the adult Drosophila retina using semithin plastic section / Konstantin Gaengel and Marek Mlodzik
- Fluorescent in situ hybridization protocols in Drosophila embryos and tissues / Eric Lécuyer, Neela Parthasarathy, and Henry M. Krause
- Linear RNA amplification for the production of microarray hybridization probes / Ansgar Klebes and Thomas B. Kornberg
- An overview of the identification, detection, and functional analysis of Drosophila microRNAs / Nicholas S. Sokol
- RNAi screening in cultured Drosophila cells / Sandra Steinbrink and Michael Boutros
- Methods for homologous recombination in Drosophila / Keith A. Maggert, Wei J. Gong, and Kent G. Golic
- Recombinases and their use in gene activation, gene inactivation, and transgenesis / Johannes Bischof and Konrad Basler
- Cuticle preparation of Drosophila embryos and larvae / Cyrille Alexandre
- Immunolabeling of embryos / H.-Arno J. Müller
- Imaging cellular and molecular dynamics in live embryos using fluorescent proteins / Matthieu Cavey and Thomas Lecuit
- Analysis of neuromuscular junctions : histology and in vivo imaging / Andreas Schmid and Stephan J. Sigrist