Adhesion protein protocols
The second edition of Adhesion Protein Protocols combines traditional techniques with cutting-edge and novel techniques that can be adapted easily to different molecules and cell types. The topics discussed in this updated second edition include novel techniques for studying cell-cell adhesion, neut...
| Other Authors: | |
|---|---|
| Format: | Book |
| Language: | English |
| Published: |
Totowa, N.J. :
Humana Press,
[2007], ©2007
Totowa, N.J. : c2007 Totowa, NJ : c2007 Totowa, N.J. : [2007] |
| Edition: | 2nd ed |
| Series: | Methods in molecular biology (Clifton, N.J.) ;
v. 370 Methods in molecular biology (Clifton, N.J.) 370 |
| Subjects: |
| LEADER | 15074nam a22014054a 4500 | ||
|---|---|---|---|
| 001 | 3d2217f3-bce3-444b-abad-831016f5fe08 | ||
| 005 | 20240903000000.0 | ||
| 008 | 061013t20072007njua b 001 0 eng | ||
| 010 | |a 2006034299 | ||
| 010 | |a 2006034299 | ||
| 010 | |a ^^2006034299 | ||
| 016 | 7 | |a 101289873 |2 DNLM | |
| 020 | |a 1588295338 (alk. paper) | ||
| 020 | |a 1588295338 |q alkaline paper | ||
| 020 | |a 9781588295330 (alk. paper) | ||
| 020 | |a 9781588295330 |q alkaline paper | ||
| 029 | 1 | |a NLM |b 101289873 | |
| 029 | 1 | |a YDXCP |b 2436346 | |
| 035 | |a (NNC)6127843 | ||
| 035 | |a (NcD)003965836DUK01 | ||
| 035 | |a (NjP)5152032-princetondb | ||
| 035 | |a (OCoLC)73502833 |z (OCoLC)973236600 |z (OCoLC)1027945737 | ||
| 035 | |a (OCoLC)73502833 | ||
| 035 | |a (OCoLC)ocm73502833 | ||
| 035 | |a (OCoLC)ocn689809786 | ||
| 035 | |a (PU)4115250-penndb-Voyager | ||
| 035 | |a 6020018 | ||
| 035 | |a 6127843 | ||
| 035 | |a 73502833 | ||
| 035 | |a 7816402 | ||
| 035 | |z (NjP)Voyager5152032 | ||
| 040 | |a DNLM/DLC |b eng |c DLC |d BAKER |d NLM |d BTCTA |d C#P |d NjP | ||
| 040 | |a DNLM/DLC |b eng |c DLC |d BAKER |d NLM |d BTCTA |d C#P |d YDXCP |d COU |d AGL |d NDK | ||
| 040 | |a DNLM/DLC |b eng |c DLC |d BAKER |d NLM |d BTCTA |d C#P |d YDXCP | ||
| 040 | |a DNLM/DLC |c DLC |d BAKER |d NLM |d BTCTA |d C#P |d YDXCP |d COU |d UtOrBLW | ||
| 040 | |a DNLM/DLC |c DLC |d BAKER |d NLM |d BTCTA |d C#P | ||
| 040 | |a DNLM/DLC |c DLC |d DLC |d CtY-M | ||
| 040 | |a DNLM/DLC |c DLC |d DLC | ||
| 042 | |a pcc | ||
| 049 | |a CGUA | ||
| 049 | |a PAUU | ||
| 050 | 4 | |a QH506 |b .M45 v.370 2007 | |
| 050 | 0 | 0 | |a QP552.C42 |b A347 2007 |
| 060 | 1 | 0 | |a QU 25 |b A234 2007 |
| 060 | 1 | 0 | |a W1 |b ME9616J v. 370 2007 |
| 070 | 0 | |a QP552.C42 |b A347 2007 | |
| 079 | |a ocm73502833 | ||
| 082 | 0 | 0 | |a 572/.4 |2 22 |
| 096 | |a QH506 |b M562 v.370 2007 | ||
| 245 | 0 | 0 | |a Adhesion protein protocols |
| 250 | |a 2nd ed | ||
| 250 | |a 2nd ed. / |b edited by Amanda S. Coutts | ||
| 250 | |a Second edition / |b edited by Amanda S. Coutts | ||
| 260 | |a Totowa, N.J. : |b Humana Press, |c [2007], ©2007 | ||
| 260 | |a Totowa, N.J. : |b Humana Press, |c c2007 | ||
| 260 | |a Totowa, NJ : |b Humana Press, |c c2007 | ||
| 264 | 1 | |a Totowa, N.J. : |b Humana Press, |c [2007] | |
| 264 | 4 | |c ©2007 | |
| 300 | |a x, 234 p. : |b ill. (some col.) ; |c 24 cm | ||
| 300 | |a x, 234 p. : |b ill. ; |c 24 cm | ||
| 300 | |a x, 234 pages : |b illustrations (some color) ; |c 24 cm | ||
| 336 | |a text |b txt |2 rdacontent | ||
| 337 | |a unmediated |b n |2 rdamedia | ||
| 338 | |a volume |b nc |2 rdacarrier | ||
| 490 | 1 | |a Methods in molecular biology, |x 1064-3745 ; |v 370 | |
| 500 | |a Earlier edition published 1999 | ||
| 500 | |a This WorldCat-derived record is shareable under Open Data Commons ODC-BY, with attribution to OCLC |5 CTY | ||
| 504 | |a Includes bibliographical references and index | ||
| 505 | 0 | |a Manipulation of cell-cell adhesion using bowtie-shaped microwells / Celeste M. Nelson, Wendy F. Liu, and Christopher S. Chen -- Separation of cell-cell adhesion complexes by differential centrifugation / Roger Vogelmann and W. James Nelson -- Analysis of neutrophil chemotaxis / Paul A. Nuzzi, Mary A. Lokuta, and Anna Huttenlocher -- Analysis of leukocyte migration through monolayers of cultured endothelial cells / Helen M. McGettrick, Lynn M. Butler, and Gerard B. Nash -- Biochemical purification of pseudopodia from migratory cells / Yingchung Wang and Richard L. Klemke -- Dynamic assessment of cell-matrix mechanical interactions in three-dimensional culture / W. Matthew Petroll -- Quantitative analyses of cell adhesion strength / Nathan D. Gallant and Andrés J. García -- Using RNA interference to knock down the adhesion protein TES / Elen Griffith -- Assaying calpain activity / Neil O. Carragher -- Analysis of focal adhesions and cytoskeleton by custom microarray / Matthew J. Dalby and Stephen J. Yarwood -- Proteomic analysis of cell suface membrane proteins in leukemic cells / Robert S. Boyd, Martin J.S. Dyer, and Kelvin Cain -- Bioinformatic analysis of adhesion proteins / Josephine C. Adams and Juergen Engel -- Analysis of integrin dynamics by fluorescence recovery after photobleaching / Bernhard Wehrle-Haller -- Double-hydrogel substrate as a model system for three-dimensional cell culture / Karen A. Beningo and Yu-li Wang -- In vitro actin assembly assays and purification from acanthamoeba / J. Bradley Zuchero | |
| 505 | 0 | |a Manipulation of cell-cell adhesion using bowtie-shaped microwells / Celeste M. Nelson, Wendy F. Liu, and Christopher S. Chen -- Separation of cell-cell adhesion complexes by differential centrifugation / Roger Vogelmann and W. James Nelson -- Analysis of neutrophil chemotaxis / Paul A. Nuzzi, Mary A. Lokuta, and Anna Huttenlocher -- Analysis of leukocyte migration through monolayers of cultured endothelial cells / Helen M. McGettrick, Lynn M. Butler, and Gerard B. Nash -- Biochemical purification of pseudopodia from migratory cells / Yingchung Wang and Richard L. Klemke -- Dynamic assessment of cell-matrix mechanical interactions in three-dimensional culture / W. Matthew Petroll -- Quantitative analyses of cell adhesion strength / Nathan D. Gallant and Andrés J. García -- Using RNA interference to knock down the adhesion protein TES / Elen Griffith -- Assaying calpain activity / Neil O. Carragher -- Analysis of focal adhesions and cytoskeleton by custom microarray / Matthew J. Dalby and Stephen J. Yarwood -- Proteomic analysis of cell surface membrane proteins in leukemic cells / Robert S. Boyd, Martin J.S. Dyer, and Kelvin Cain -- Bioinformatic analysis of adhesion proteins / Josephine C. Adams and Juergen Engel -- Analysis of integrin dynamics by fluorescence recovery after photobleaching / Bernhard Wehrle-Haller -- Double-hydrogel substrate as a model system for three-dimensional cell culture / Karen A. Beningo and Yu-li Wang -- In vitro actin assembly assays and purification from acanthamoeba / J. Bradley Zuchero | |
| 505 | 0 | 0 | |g 1 |t Manipulation of Cell-Cell Adhesion Using Bowtie-Shaped Microwells / |r Celeste M. Nelson, Wendy F. Liu, Christopher S. Chen |g 1 -- |g 2 |t Separation of Cell-Cell Adhesion Complexes By Differential Centrifugation / |r Roger Vogelmann, W. James Nelson |g 11 -- |g 3 |t Analysis of Neutrophil Chemotaxis / |r Paul A. Nuzzi, Mary A. Lokuta, Anna Huttenlocher |g 23 -- |g 4 |t Analysis of Leukocyte Migration Through Monolayers of Cultured Endothelial Cells / |r Helen M. McGettrick, Lynn M. Butler, Gerard B. Nash |g 37 -- |g 5 |t Biochemical Purification of Pseudopodia From Migratory Cells / |r Yingchun Wang, Richard L. Klemke |g 55 -- |g 6 |t Dynamic Assessment of Cell-Matrix Mechanical Interactions in Three-Dimensional Culture / |r W. Matthew Petroll |g 67 -- |g 7 |t Quantitative Analyses of Cell Adhesion Strength / |r Nathan D. Gallant, Andreʹs J. Garciʹa |g 83 -- |g 8 |t Using RNA Interference to Knock Down the Adhesion Protein TES / |r Elen Griffith |g 97 -- |g 9 |t Assaying Calpain Activity / |r Neil O. Carragher |g 109 -- |g 10 |t Analysis of Focal Adhesions and Cytoskeleton by Custom Microarray / |r Matthew J. Dalby, Stephen J. Yarwood |g 121 -- |g 11 |t Proteomic Analysis of Cell Surface Membrane Proteins in Leukemic Cells / |r Robert S. Boyd, Martin J. S. Dyer, Kelvin Cain |g 135 -- |g 12 |t Bioinformatic Analysis of Adhesion Proteins / |r Josephine C. Adams, Juergen Engel |g 147 -- |g 13 |t Analysis of Integrin Dynamics by Fluorescence Recovery After Photobleaching / |r Bernhard, Wehrle-Haller |g 173 -- |g 14 |t Double-Hydrogel Substrate as a Model System for Three-Dimensional Cell Culture / |r Karen A. Beningo, Yu-li Wang |g 203 -- |g 15 |t In Vitro Actin Assembly Assays and Purification From Acanthamoeba / |r J. Bradley Zuchero |g 213 |
| 520 | |a The second edition of Adhesion Protein Protocols combines traditional techniques with cutting-edge and novel techniques that can be adapted easily to different molecules and cell types. The topics discussed in this updated second edition include novel techniques for studying cell-cell adhesion, neutrophil chemotaxis, in vitro assays used to study leukocyte migration through monolayers of cultured endothelial cells, and novel techniques to purify pseudopodia from migratory cells. This book also discusses the study of cell-matrix interaction, RNA interference, fluorescence recovery after photobleaching, actin purification, the application of microarray techniques, and the role of adhesion proteins in the study of proteomics | ||
| 520 | |a "The second edition of Adhesion Protein Protocols combines traditional techniques with cutting-edge and novel techniques that can be adapted easily to different molecules and cell types. The topics discussed in this updated second edition include novel techniques for studying cell-cell adhesion, neutrophil chemotaxis, in vitro assays used to study leukocyte migration through monolayers of cultured endothelial cells, and novel techniques to purify pseudopodia from migratory cells. This book also discusses the study of cell-matrix interaction, RNA interference, fluorescence recovery after photobleaching, actin purification, the application of microarray techniques, and the role of adhesion proteins in the study of proteomics. The protocols discussed in this volume are suitable for both novice and expert scientists, who will gain further insight into the complex and incompletely understood processes involved in cellular adhesion."--Publisher's website | ||
| 520 | 8 | |a Adhesion protein proteomics | |
| 520 | 8 | |a Contents | |
| 520 | 8 | |a Features | |
| 520 | 8 | |a Manipulation of Cell-Cell Adhesion Using Bowtie-Shaped Microwells. Separation of Cell-Cell Adhesion Complexes By Differential Centrifugation. Analysis of Neutrophil Chemotaxis. Analysis of Leukocyte Migration Through Monolayers of Cultured Endothelial Cells. Biochemical Purification of Pseudopodia From Migratory Cells. Dynamic Assessment of Cell-Matrix Mechanical Interactions in Three-Dimensional Culture. Quantitative Analyses of Cell Adhesion Strength. Using RNA Interference to Knock Down the Adhesion Protein TES. Assaying Calpain Activity. Analysis of Focal Adhesions and Cytoskeleton by Custom Microarray. Proteomic Analysis of Cell Surface Membrane Proteins in Leukemic Cells. Bioinformatic Analysis of Adhesion Proteins. Analysis of Integrin Dynamics by Fluorescence Recovery After Photobleaching. Double-Hydrogel Substrate as a Model System for Three-Dimensional Cell Culture. In Vitro Actin Assembly Assays and Purification From Acanthamoeba. Index | |
| 520 | 8 | |a Novel techniques for studying cell-cell adhesion | |
| 520 | 8 | |a The latest methods for laboratory use of microarray techniques | |
| 520 | 8 | |a The protocols discussed in this volume are suitable for both novice and expert scientists, who will gain further insight into the complex and incompletely understood processes involved in cellular adhesion | |
| 530 | |a Also available in an electronic version | ||
| 530 | |a Also available via the World Wide Web | ||
| 650 | 0 | |a Cell adhesion molecules |v Laboratory manuals |0 https://id.loc.gov/authorities/subjects/sh99001586 | |
| 650 | 0 | |a Cell adhesion molecules |v Laboratory manuals | |
| 650 | 7 | |a Cell adhesion molecules |2 fast | |
| 650 | 1 | 2 | |a Cell Adhesion |v Laboratory Manuals |
| 650 | 1 | 2 | |a Cell Adhesion |
| 650 | 2 | 2 | |a Cell Adhesion Molecules |v Laboratory Manuals |
| 650 | 2 | 2 | |a Cell Adhesion Molecules |
| 650 | 2 | 2 | |a Molecular Biology |x methods |
| 655 | 2 | |a Laboratory Manual |0 http://id.nlm.nih.gov/mesh/D020484 | |
| 655 | 2 | |a Laboratory Manual | |
| 655 | 7 | |a Aufsatzsammlung |2 swd | |
| 655 | 7 | |a Handbooks and manuals |2 fast | |
| 655 | 7 | |a Handbooks, manuals, etc |2 fast | |
| 655 | 7 | |a Laboratory manuals |2 fast |0 http://id.worldcat.org/fast/1920776 | |
| 655 | 7 | |a Laboratory manuals |2 fast | |
| 655 | 7 | |a Laboratory manuals |2 lcgft | |
| 700 | 1 | |a Coutts, Amanda S |0 http://viaf.org/viaf/29864719 | |
| 700 | 1 | |a Coutts, Amanda S |1 http://viaf.org/viaf/29864719 | |
| 700 | 1 | |a Coutts, Amanda S | |
| 830 | 0 | |a Methods in molecular biology (Clifton, N.J.) ; |v v. 370 | |
| 830 | 0 | |a Methods in molecular biology (Clifton, N.J.) |v 370 | |
| 999 | 1 | 0 | |i 3d2217f3-bce3-444b-abad-831016f5fe08 |l 7816402 |s US-CTY |m adhesion_protein_protocols_________________________________________________2007____2__humana___________________________________________________________________________p |
| 999 | 1 | 0 | |i 3d2217f3-bce3-444b-abad-831016f5fe08 |l 6329211 |s US-ICU |m adhesion_protein_protocols_________________________________________________2007____2__humana___________________________________________________________________________p |
| 999 | 1 | 0 | |i 3d2217f3-bce3-444b-abad-831016f5fe08 |l 990102731950203941 |s US-MH |m adhesion_protein_protocols_________________________________________________2007____2__humana___________________________________________________________________________p |
| 999 | 1 | 0 | |i 3d2217f3-bce3-444b-abad-831016f5fe08 |l 990039658360108501 |s US-NCD |m adhesion_protein_protocols_________________________________________________2007____2__humana___________________________________________________________________________p |
| 999 | 1 | 0 | |i 3d2217f3-bce3-444b-abad-831016f5fe08 |l 6020018 |s US-NIC |m adhesion_protein_protocols_________________________________________________2007____2__humana___________________________________________________________________________p |
| 999 | 1 | 0 | |i 3d2217f3-bce3-444b-abad-831016f5fe08 |l 9951520323506421 |s US-NJP |m adhesion_protein_protocols_________________________________________________2007____2__humana___________________________________________________________________________p |
| 999 | 1 | 0 | |i 3d2217f3-bce3-444b-abad-831016f5fe08 |l 6127843 |s US-NNC |m adhesion_protein_protocols_________________________________________________2007____2__humana___________________________________________________________________________p |
| 999 | 1 | 0 | |i 3d2217f3-bce3-444b-abad-831016f5fe08 |l 9941152503503681 |s US-PU |m adhesion_protein_protocols_________________________________________________2007____2__humana___________________________________________________________________________p |
| 999 | 1 | 1 | |l 7816402 |s ISIL:US-CTY |t BKS |a lsfmed |b 39002079438710 |c QP552 C42 A347 2007 |g 0 |v 1 piece |x lsfc |y 7063733 |p LOANABLE |
| 999 | 1 | 1 | |l 6329211 |s ISIL:US-ICU |t BKS |a ASR-SciASR |b A76144724 |c QH506.M45 v.370 |d Library of Congress classification |y 8188611 |p LOANABLE |
| 999 | 1 | 1 | |l 990102731950203941 |s ISIL:US-MH |t BKS |a MED GEN |b 32044073357725 |c QU 55.3 A2352 2007 |d 2 |x 01 BOOK |y 232103027980003941 |p LOANABLE |
| 999 | 1 | 1 | |l 990039658360108501 |s ISIL:US-NCD |t BKS |a MCL MSTKS |b M00364369V |c QH506 M562 v.370 2007 |d 2 |x BOOK |y 23752880340008501 |p UNLOANABLE |
| 999 | 1 | 1 | |l 6020018 |s ISIL:US-NIC |t BKS |a mann |b 31924105299642 |c QP552.C42 A347 2007 |d lc |k 1 |x Book |y d79d600d-6270-458c-ba29-19b4c4393f9c |p LOANABLE |
| 999 | 1 | 1 | |l 9951520323506421 |s ISIL:US-NJP |t BKS |a lewis serial |b 32101061359517 |c QH506 .M45 1984 vol.370 |d 0 |x NoCirc |y 23531738230006421 |p UNLOANABLE |
| 999 | 1 | 1 | |l 6127843 |s ISIL:US-NNC |t BKS |a off,hsl |b HS00345709 |c QH506 .M56 v.370 |y 6146643 |p LOANABLE |
| 999 | 1 | 1 | |l 6127843 |s ISIL:US-NNC |t BKS |a sci |b 0067721770 |c QP552.C42 A347 2007 |y 6152817 |p LOANABLE |
| 999 | 1 | 1 | |l 9941152503503681 |s ISIL:US-PU |t BKS |a BiomLib biom |b 31198045036709 |c QH506 .M45 v.370 2007 |d 0 |x BOOK |y 23279017510003681 |p UNLOANABLE |