Morphology methods : cell and molecular biology techniques /

This text explores the most significant aspects of sophisticated and recent cell biological techniques that are needed to keep up with research in morphology. Researchers in morphology will find many step-by-step protocols to conduct their work including state-of -- the -- art laser capture microdis...

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Bibliographic Details
Other Authors:	Lloyd, Ricardo V
Format:	Book
Language:	English
Published:	Totowa, N.J. : Humana Press, c2001 Totowa, NJ : 2001 Totowa, NJ : c2001 Totowa, N.J. : 2001
Subjects:	Cytology > Technique Fluorescence in situ hybridization Immunohistochemistry Molecular biology > Technique Polymerase chain reaction Cytological Techniques Immunohistochemistry > methods In Situ Hybridization, Fluorescence > methods Molecular Biology > methods Polymerase Chain Reaction > methods Polymerase Chain Reaction Molecular Biology > methods > Laboratory Manuals Cytological Techniques > methods > Laboratory Manuals Cytological Techniques > methods Immunohistochemistry > methods > Laboratory Manuals In Situ Hybridization > methods Nucleic Acids > analysis > Laboratory Manuals Nucleic Acids > analysis Situ Hybridization > methods Laboratory Manual

Table of Contents:

1 Introduction to Molecular Methods / Ricardo V. Lloyd
2. Laser Capture Microdissection: Principles and Applications / Ricardo V. Lloyd
3. In Situ Hybridization: Detection of DNA and RNA / Long Jin, Xiang Qian and Ricardo V. Lloyd
4. In Situ Detection of Infectious Agents / Gary W. Procop and Randall Hayden
5. Fluorescent In Situ Hybridization / Susan Sheldon
6. Practical Applications of the FISH Technique / George Kontogeorgos, Nikiforos Kapranos and Eleni Thodou
7. Tyramide Amplification Methods for In Situ Hybridization / John L. Frater and Raymond R. Tubbs
8. Protocols: Ultrastructural In Situ Hybridization / Akira Matsuno, Tadashi Nagashima and R. Yoshiyuki Osamura / [et al.]
9. Quantitation of In Situ Hybridization Analysis / Lars-Inge Larsson
10. Protocols: Confocal Laser Scanning Microscopy / Akira Matsuno, Johbu Itoh and Tadashi Nagashima / [et al.]
11. Protocols: Polymerase Chain Reaction / Yuri E. Nikiforov and Philip N. Howles
12. Protocols: Detection of Nucleic Acids in Cells and Tissues by In Situ Polymerase Chain Reaction / Omar Bagasra, Lisa E. Bobroski and Muhammad Amjad
13. Immunohistochemistry: Theory and Practice / Patrick C. Roche and Eric D. Hsi
14. Antigen Retrieval Technique for Immunohistochemistry: Principles, Protocols, and Further Development / Clive R. Taylor and Shan-Rong Shi
15. Tyramide Amplification in Immunohistochemistry / Naoko Sanno, Akira Teramoto and R. Yoshiyuki Osamura
16. Application of Immunohistochemistry in the Diagnosis of Lymphoid Lesions / Paul J. Kurtin
17. Applications of Immunohistochemistry in the Diagnosis of Undifferentiated Tumors / Mark R. Wick and Lisa A. Cerilli
18. Applications of Immunohistochemistry in the Diagnosis of Endocrine Lesions / Ricardo V. Lloyd
19. Ultrastructural Immunohistochemistry / Sergio Vidal, Eva Horvath and Kalman Kovacs
20. Protocols: Clonality Analysis / Aurel Perren and Paul Komminoth.
1 Introduction to Molecular Methods / Ricardo V. Lloyd
2. Laser Capture Microdissection: Principles and Applications / Ricardo V. Lloyd
3. In Situ Hybridization: Detection of DNA and RNA / Long Jin, Xiang Qian and Ricardo V. Lloyd
4. In Situ Detection of Infectious Agents / Gary W. Procop and Randall Hayden
5. Fluorescent In Situ Hybridization / Susan Sheldon
6. Practical Applications of the FISH Technique / George Kontogeorgos, Nikiforos Kapranos and Eleni Thodou
7. Tyramide Amplification Methods for In Situ Hybridization / John L. Frater and Raymond R. Tubbs
8. Protocols: Ultrastructural In Situ Hybridization / Akira Matsuno, Tadashi Nagashima and R. Yoshiyuki Osamura / [et al.]
9. Quantitation of In Situ Hybridization Analysis / Lars-Inge Larsson
10. Protocols: Confocal Laser Scanning Microscopy / Akira Matsuno, Johbu Itoh and Tadashi Nagashima / [et al.]
11. Protocols: Polymerase Chain Reaction / Yuri E. Nikiforov and Philip N. Howles
12. Protocols: Detection of Nucleic Acids in Cells and Tissues by In Situ Polymerase Chain Reaction / Omar Bagasra, Lisa E. Bobroski and Muhammad Amjad
13. Immunohistochemistry: Theory and Practice / Patrick C. Roche and Eric D. Hsi
14. Antigen Retrieval Technique for Immunohistochemistry: Principles, Protocols, and Further Development / Clive R. Taylor and Shan-Rong Shi
15. Tyramide Amplification in Immunohistochemistry / Naoko Sanno, Akira Teramoto and R. Yoshiyuki Osamura
16. Application of Immunohistochemistry in the Diagnosis of Lymphoid Lesions / Paul J. Kurtin
17. Applications of Immunohistochemistry in the Diagnosis of Undifferentiated Tumors / Mark R. Wick and Lisa A. Cerilli
18. Applications of Immunohistochemistry in the Diagnosis of Endocrine Lesions / Ricardo V. Lloyd
19. Ultrastructural Immunohistochemistry / Sergio Vidal, Eva Horvath and Kalman Kovacs
1 Introduction to Molecular Methods / Ricardo V. Lloyd
2. Laser Capture Microdissection: Principles and Applications / Ricardo V. Lloyd
3. In Situ Hybridization: Detection of DNA and RNA / Long Jin, Xiang Qian and Ricardo V. Lloyd
4. In Situ Detection of Infectious Agents / Gary W. Procop and Randall Hayden
5. Fluorescent In Situ Hybridization / Susan Sheldon
6. Practical Applications of the FISH Technique / George Kontogeorgos, Nikiforos Kapranos and Eleni Thodou
7. Tyramide Amplification Methods for In Situ Hybridization / John L. Frater and Raymond R. Tubbs
8. Protocols: Ultrastructural In Situ Hybridization / Akira Matsuno, Tadashi Nagashima and R. Yoshiyuki Osamura / [et al.]
9. Quantitation of In Situ Hybridization Analysis / Lars-Inge Larsson
10. Protocols: Confocal Laser Scanning Microscopy / Akira Matsuno, Johbu Itoh and Tadashi Nagashima / [et al.]
2 Laser Capture Microdissection: Principles and Applications / Ricardo V. Lloyd 11
Protocol: H&E Staining 23
Protocol: Operating the PIX Cell System 24
Protocol: DNA Extraction from Collected Cells 24
Protocol: RNA Extraction from Collected Cells 25
3 In Situ Hybridization: Detection of DNA and RNA / Long Jin, Xiang Qian, Ricardo V. Lloyd 27
Protocol: In Situ Hybridization 45
4 In Situ Detection of Infectious Agents / Gary W. Procop, Randall Hayden 47
Protocol: Detecting Adenovirus 61
Protocol: Epstein-Barr Virus (Eber I/II) 63
5 Fluorescent In Situ Hybridization / Susan Sheldon 67
Protocol: FISH 84
6 Practical Applications of the FISH Technique / George Kontogeorgos, Nikiforos Kapranos, Eleni Thodou 91
Protocol: Metaphase Analysis from Whole Blood Culture 102
Protocol: Metaphase Analysis from Solid Tumors 106
Protocol: Interphase Analysis on Cell Imprints--Cytology Specimens 108
Protocol: Interphase Analysis of Formalin-Fixed Paraffin-Embedded Tissues 110
7 Tyramide Amplification Methods for In Situ Hybridization / John L. Frater, Raymond R. Tubbs 113
Protocol: TSA by Rapp et al., 1995 125
Protocol: CARD/TSA with Nanogold 126
Protocol: Tyramide-enhanced FISH with Peroxidase-labeled Oligonucleotides 127
8 Protocols: Ultrastructural In Situ Hybridization / Akira Matsuno, Tadashi Nagashima, R. Yoshiyuki Osamura, Keiichi Watanabe 129
9 Quantitation of In Situ Hybridization Analysis / Lars-Inge Larsson 145
10 Protocols: Confocal Laser Scanning Microscopy / Akira Matsuno, Johbu Itoh, Tadashi Nagashima, R. Yoshiyuki Osamura, Keiichi Watanabe 165
11 Protocols: Polymerase Chain Reaction / Yuri E. Nikiforov, Philip N. Howles 181
12 Protocols: Detection of Nucleic Acids in Cells and Tissues by In Situ Polymerase Chain Reaction / Omar Bagasra, Lisa E. Bobroski, Muhammad Amjad 209
13 Immunohistochemistry: Theory and Practice / Patrick C. Roche, Eric D. Hsi 229
14 Antigen Retrieval Technique for Immunohistochemistry: Principles, Protocols, and Further Development / Clive R. Taylor, Shan-Rong Shi 239
Protocol: Antigen Retrieval (AR) Technique 260
15 Tyramide Amplification in Immunohistochemistry / Naoko Sanno, Akira Teramoto, R. Yoshiyuki Osamura 267
Protocol: CARD Immunohistochemistry 276
16 Application of Immunohistochemistry in the Diagnosis of Lymphoid Lesions / Paul J. Kurtin 277
17 Applications of Immunohistochemistry in the Diagnosis of Undifferentiated Tumors / Mark R. Wick, Lisa A. Cerilli 323
18 Applications of Immunohistochemistry in the Diagnosis of Endocrine Lesions / Ricardo V. Lloyd 361
19 Ultrastructural Immunohistochemistry / Sergio Vidal, Eva Horvath, Kalman Kovacs 375
Protocol: Ultrastructural Immunohistochemistry 399
20 Protocols: Clonality Analysis / Aurel Perren, Paul Komminoth 403
Introduction to Molecular Methods / Ricardo V. Lloyd
Laser Capture Microdissection: Principles and Applications / Ricardo V. Lloyd
Protocol: HandE Staining
Protocol: Operating the PIX Cell System
Protocol: DNA Extraction from Collected Cells
Protocol: RNA Extraction from Collected Cells
In Situ Hybridization: Detection of DNA and RNA / Long Jin, Xiang Qian, Ricardo V. Lloyd
Protocol: In Situ Hybridization
In Situ Detection of Infectious Agents / Gary W. Procop, Randall Hayden
Protocol: Detecting Adenovirus
Protocol: Epstein-Barr Virus (Eber I/II)
Fluorescent In Situ Hybridization / Susan Sheldon
Protocol: FISH
Practical Applications of the FISH Technique / George Kontogeorgos, Nikiforos Kapranos, Eleni Thodou
Protocol: Metaphase Analysis from Whole Blood Culture
Protocol: Metaphase Analysis from Solid Tumors
Protocol: Interphase Analysis on Cell Imprints--Cytology Specimens
Protocol: Interphase Analysis of Formalin-Fixed Paraffin-Embedded Tissues
Tyramide Amplification Methods for In Situ Hybridization / John L. Frater, Raymond R. Tubbs
Protocol: TSA by Rapp et al., 1995
Protocol: CARD/TSA with Nanogold
Protocol: Tyramide-enhanced FISH with Peroxidase-labeled Oligonucleotides
Protocols: Ultrastructural In Situ Hybridization / Akira Matsuno, Tadashi Nagashima, R. Yoshiyuki Osamura, Keiichi Watanabe
Quantitation of In Situ Hybridization Analysis / Lars-Inge Larsson
11 Protocols: Polymerase Chain Reaction / Yuri E. Nikiforov and Philip N. Howles
12. Protocols: Detection of Nucleic Acids in Cells and Tissues by In Situ Polymerase Chain Reaction / Omar Bagasra, Lisa E. Bobroski and Muhammad Amjad
13. Immunohistochemistry: Theory and Practice / Patrick C. Roche and Eric D. Hsi
14. Antigen Retrieval Technique for Immunohistochemistry: Principles, Protocols, and Further Development / Clive R. Taylor and Shan-Rong Shi
15. Tyramide Amplification in Immunohistochemistry / Naoko Sanno, Akira Teramoto and R. Yoshiyuki Osamura
16. Application of Immunohistochemistry in the Diagnosis of Lymphoid Lesions / Paul J. Kurtin
17. Applications of Immunohistochemistry in the Diagnosis of Undifferentiated Tumors / Mark R. Wick and Lisa A. Cerilli
18. Applications of Immunohistochemistry in the Diagnosis of Endocrine Lesions / Ricardo V. Lloyd
19. Ultrastructural Immunohistochemistry / Sergio Vidal, Eva Horvath and Kalman Kovacs
20 Protocols: Clonality Analysis / Aurel Perren and Paul Komminoth.

Morphology methods : cell and molecular biology techniques /

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